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Mostueatecine A and B: Two Indole Alkaloids and Mostueatecine C, One Triterpene from Mostuea batesii
Gompe Eric Gustave,
Ouahouo Wache Blandine Marlyse,
Mkounga Pierre,
Tabouguia Merveille,
Assob Nguedia Jules,
Clement Fotie Jean,
Nkengfack Augustin Ephrem
Issue:
Volume 2, Issue 6, December 2016
Pages:
60-66
Received:
19 July 2016
Accepted:
25 August 2016
Published:
3 November 2016
Abstract: Two new indole alkaloid derivatives namely Mostueatecine A (1), Mostueatecine B (2) and one new triterpene derivative Mostueatecine C (3) were isolated from the stem and leaves of Mostuea batesii, along with five known compounds: Camptothecine (4), β-amyrin-3-O-glucopyranoside (5), Oleanolic acid (6), 2α,3α,19α-trihydroxy-24-norurs-4(23),12-dien-28-oic acid (7) and Saccharose(8). The structures of these compounds were elucidated using a detailed analysis of their HRESI-MS, 1D and 2D NMR spectroscopic data. Some of these compounds were evaluated in vitro for their antimicrobial activities against a wide range of microorganisms. The results showed that none of them possess noticeable activity.
Abstract: Two new indole alkaloid derivatives namely Mostueatecine A (1), Mostueatecine B (2) and one new triterpene derivative Mostueatecine C (3) were isolated from the stem and leaves of Mostuea batesii, along with five known compounds: Camptothecine (4), β-amyrin-3-O-glucopyranoside (5), Oleanolic acid (6), 2α,3α,19α-trihydroxy-24-norurs-4(23),12-dien-28...
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Evaluation of the Antidiabetic Properties of Hydro-Alcoholic Extract and Its Fractions from Physalis peruviana L. Leaves on Streptozotocin-Induced Diabetic Wistar Rats
Felicien M. Kasali,
Charles Ntungwen Fokunang,
Joseph Ngoupayo,
Estella Tembe-Fokunang,
Bathelemy Ngameni,
Borgia Njinkio,
Frederick Kechia,
Justin N. Kadima,
Tsague Marthe,
Oyono Victor,
Wilfred Mbacham,
Bonaventure Tchaleu Ngadjui
Issue:
Volume 2, Issue 6, December 2016
Pages:
67-73
Received:
10 July 2016
Accepted:
20 July 2016
Published:
22 November 2016
Abstract: The purpose of this study was to investigate the antidiabetic activity of extracts from leaves of Physalis peruviana L. used in the Eastern part of the Democratic Republic of the Congo used against diabetes. Different fractions with hexane, ethyl acetate and residue were obtained from the hydroalcoholic extract of Physalis peruviana leaves. The antidiabetic evaluation of hydroalcoholic and its fractions was evaluated in diabetic rats by a single administration of streptozotocin (50 mg/kg body weight) intravenously. The Reference group received glibenclamide (6.5 mg/kg body weight) and each test group received 100 mg/kg of body weight. Those groups were compared with a control group which received only a Tween 20 solution (1 ml per 100 g body weight). Serum biochemical profiles were evaluated by some blood markers including serum glucose, serum alanine transaminase (ALT), serum aspartate transaminase (AST) serum creatinine, total protein, triglyceride, total cholesterol, HDL-cholesterol and LDL-cholesterol. Antidiabetic profile evaluation showed no significant variation (P < 0.05) in blood glucose between groups after 28 days of treatment. There was no significant difference in the biochemical markers change including creatinine, ALT, AST, total protein, triglycerides, total cholesterol, HDL-cholesterol and LDL-cholesterol. The hydroalcoholic extract from the leaves of Physalis peruviana L. and its fractions showed antidiabetic activity suggesting future detailed studies for new chemical entities lead drug discovery.
Abstract: The purpose of this study was to investigate the antidiabetic activity of extracts from leaves of Physalis peruviana L. used in the Eastern part of the Democratic Republic of the Congo used against diabetes. Different fractions with hexane, ethyl acetate and residue were obtained from the hydroalcoholic extract of Physalis peruviana leaves. The ant...
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Identification of the Bioactive Compounds Hypotensive Effect in the Ethyl Acetate Extract of Eribroma oblongum (Malvaceae) Stem Bark
Tsague M. V.,
Fokunang Ntungwen C.,
Talla E.,
Djekilamber A.,
Tembe-Fokunang E. A.,
Ngo Lemba Tom E.,
Essomba C.,
Ntchapda F.,
Sokeng Dongmo S.,
Oben Enyong J.,
Ze Minkande J.,
Afane Ela A.,
Temdie Guemmogne R. J.,
Dimo T.,
Chi Fru G.,
Ngadjui Tchaleu B.
Issue:
Volume 2, Issue 6, December 2016
Pages:
74-82
Received:
6 October 2016
Accepted:
14 October 2016
Published:
21 November 2016
Abstract: The stem bark of Eribroma oblongum (malvaceae) is used in traditional Cameroonian medicine to treat various metabolic illnesses including the management of hypertension but there is no scientific evidence to how relief is brought about. The present study was to evaluate the effect of the ethyl acetate extract of the dried stem bark of E. oblongum on arterial blood pressure and heart rate in normotensive rat (NTR) and their mechanisms of EAEO. The effects of ethyl acetate extract of Eribroma oblongum (EAEO; 10, 20, 30 mg/kg; i.v) was tested on systolic blood pressure (SBP) and heart rate (HR) of normotensive rat. The mechanism of EAEO (20mg/kg) was studied in the presence of atropine, yohimbine, propranolol, L-NAME or reserpine. At the end of the experiment, SBP and HR were recorded. EAEO (10-20 mg/kg) induced a significant hypotensive effect of SBP. The hypotensive effects of EAEO (20 mg/kg) were inhibited by pre-treatment of rats with atropine, reserpine, yohimbine and L-NAME. At the end of this study the result demonstrates that the hypotensive as well as the antihypertensive effects of the ethyl acetate extract of the stem bark of Eribroma oblongum. Our data validate the use of the extract in traditional medicine against hypertension. The effect on blood pressure is, at least in part, due to a modulation of the orthosympathetic nervous system and to the improvement of the antioxidant status. Further studies are from now needed to study the toxicity of Eribroma oblongum.
Abstract: The stem bark of Eribroma oblongum (malvaceae) is used in traditional Cameroonian medicine to treat various metabolic illnesses including the management of hypertension but there is no scientific evidence to how relief is brought about. The present study was to evaluate the effect of the ethyl acetate extract of the dried stem bark of E. oblongum o...
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Phytochemical Screening and Biological Activity Studies of the Extract from the Bark of Ricinodendron heudoletti, Euphorbiaceae
Oyono Victor,
Fokunang Charles,
Tembe-Fokunang Estella,
Tsague Marthe,
Messi Angélique,
Assam Jean,
Ngameni Bethelemy,
Kechia Frederick,
Armel Herve Nwabo Kamdje,
Penlap Veronique,
Ngadjui Bonaventure
Issue:
Volume 2, Issue 6, December 2016
Pages:
83-89
Received:
6 July 2016
Accepted:
12 July 2016
Published:
10 January 2017
Abstract: The fractions and pure compounds isolated from the bark of R. heudoletti were tested for their antibacterial properties on negative and positive gram bacteria using the disc diffusion method. Phytochemical analysis of the fractions and pure compound isolated was also conducted. The inhibitions parameters of the fractions and pure compound were determined using macro dilution method. The results showed that fraction and isoflavonoid exhibited a significant antibacterial effect against twelve strains isolated from patients. These micro-organisms were Gram-negative (Escherichia coli, Salmonella typhi, Shigella flexneri, Pseudomonas aeroginosa, Enterobacter cloacae, Klebsiella pneumoniae, Klebsiella oxytoca, Morganella morganii, Citrobacter freundi, and Proteus vulgaris) and positive gram (Staphylococcus aureus, and Streptococcus feacalis). The ratio of minimal bactericidal concentration (MBC) over the minimal inhibitory concentration (MIC) indicated a promising bactericidal effect of fractions and pure compounds isolated. These results support the current common use of the bark of Ricinodendron heudoletti and flavonoids in the treatment of some infectious diseases.
Abstract: The fractions and pure compounds isolated from the bark of R. heudoletti were tested for their antibacterial properties on negative and positive gram bacteria using the disc diffusion method. Phytochemical analysis of the fractions and pure compound isolated was also conducted. The inhibitions parameters of the fractions and pure compound were dete...
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Knowledge, Attitudes and Practices of Heath Traditional Practitioners (TP) Deal with Viral Hepatitis in Bobo-Dioulasso in Burkina Faso
Koura Mali,
Sombie Issiaka,
Serme Abdel Karim,
Napon Zongo Delphine,
Somda Sosthène,
Coulibaly Aboubacar,
Ouattara Zanga Damien,
Bamouni Sophie,
Sawadogo Appolinaire
Issue:
Volume 2, Issue 6, December 2016
Pages:
90-95
Received:
18 September 2016
Accepted:
2 December 2016
Published:
10 January 2017
Abstract: Viral hepatitis is a major public health problem worldwide and in Burkina Faso. Traditional healers are widely involved in its therapeutic management. The aim of our study was to investigate the knowledge attitudes and practices of traditional healers on viral hepatitis. This is a descriptive cross-acted study of February the 1st to April the 30th, 2015 with 100 traditional practitioners affiliated to the two associations of traditional practitioners in Bobo Dioulasso. The knowledge on viral hepatitis appeared generally unsufficient in 95%. We recorded a good level of knowledge on the definition in 57% of viral etiology in 8.8%, on the kinds of viral hepatitis in 3.5%. The main routes of contamination pathways namely sexual and blood were known to 44.4% and 11.1% respectively. The mother-child transmission was known only 5.5%. Regarding the clinical, jaundice and fever were known only to 14,03% and 3.5% respectively. Vaccination was only known to only 26.4%. Literacy has been associated with a good overall knowledge of viral hepatitis (p=0.01) as opposed to ancienty in practice (0.16). The plants are central to the therapeutic arsenal for 91.5% of TP. Most cited were Chrysantheliumamericana, Combretummicranthum, Cochlespermumtinctorium, Anogeissusleiocarpus, Terminaliamacropter. The leaves were the most used (62.5%). The form of the most common presentation was decoction (82.6%). The route of administration the most recommended was oral (91.3). Medical Analyses were the main reason for reference to modern medicine (62.5%). This study demonstrated a knowledge of TP deficiency on viral hepatitis. The knowledge was associated with literacy. The plants dominate in the armamentarium of the TP, some found in our study should be the subject of pharmacological studies.
Abstract: Viral hepatitis is a major public health problem worldwide and in Burkina Faso. Traditional healers are widely involved in its therapeutic management. The aim of our study was to investigate the knowledge attitudes and practices of traditional healers on viral hepatitis. This is a descriptive cross-acted study of February the 1st to April the 30th,...
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Role of Aged Garlic Extract Against Radiation Induced Oxidative Stress Associated with Some Biochemical Disorders in Male Albino Rats
Kouam Foubi Brice Bertrand,
Chuisseu Djamen Dieudonné Pascal,
Dzeufiet Djomeni Paul Désiré,
Mopia Foubi Myriam Arielle,
Samba Ngano Odette,
Mouelle Sone,
Tiedeu Alain Bertin,
Gonsu Fotsin Joseph
Issue:
Volume 2, Issue 6, December 2016
Pages:
96-116
Received:
19 October 2016
Accepted:
23 November 2016
Published:
11 January 2017
Abstract: This study was conducted to clarify the potential role of AGE against damages induced in rats due to exposure to gamma radiation. Adult male albino rats (214-230g). Eight groups, five healthy male rats each were used (20 irradiated and 20 Sham Irradiated), among which some were receiving via gavages distilled water, the others AGE at different doses (25 mg/kg and 50 mg/kg) and the rest vitamin E+Alpha Lipoïc Acid. Blood samples were collected at day 8 post irradiation for biochemical assay. Exposure of rats to gamma radiation caused a significant increase in the level of total cholesterol (TC), triglycerides (TG), LDL-Cholesterol, Malondialdehyde (MDA), Nitrite (NO2-), Creatinine and AST, ALT, ALP and Bilirubin (Total Serum Bilirubin, Direct Bilirubin and Unconjugated Bilirubin)while a significant decrease was recorded in HDL-Cholesterol, serum total proteins, glutathione content (GSH), superoxide dismutase (SOD), catalase (CAT) activities and total protein level in organs tissues. In rats treated with AGE then exposed to radiation, the results showed an improvement in all previous parameters. It could be concluded that AGE might reduce the biological hazards in rats induced by gamma irradiation.
Abstract: This study was conducted to clarify the potential role of AGE against damages induced in rats due to exposure to gamma radiation. Adult male albino rats (214-230g). Eight groups, five healthy male rats each were used (20 irradiated and 20 Sham Irradiated), among which some were receiving via gavages distilled water, the others AGE at different dose...
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Metabolic Effects of Goutweed (Aegopodium podagraria L.) Tincture and Metformin in Dexamethasone-Treated Rats
Issue:
Volume 2, Issue 6, December 2016
Pages:
117-126
Received:
30 October 2016
Accepted:
25 November 2016
Published:
11 January 2017
Abstract: The interest in the preclinical studies of the efficacy and safety of herbal drugs combinations with antidiabetic medicines increases. Aegopodium podagraria L. (goutweed) is a plant widely used in traditional medicine. The tincture obtained from its aerial part is characterized by antihyperglycemic, lipid-lowering, hepatoprotective and nephroprotective effects and is able to partially increase the efficacy of metformin in the animals receiving dexamethasone (permissive effect in regard to glucose and lipid metabolism normalization). The objective of this study is to determine the influence of goutweed tincture combined with metformin on protein and purine metabolism as well as the state of liver in dexamethasone-treated rats. The animals were divided into 5 groups as follows: intact control, dexamethasone (untreated), dexamethasone + metformin, 50 mg/kg; dexamethasone + A. podagraria tincture, 1 ml/kg intragastrically; dexamethasone + metformin, 50 mg/kg intragastrically + A. podagraria tincture, 1 ml/kg intragastrically. Dexamethasone was used at a dose of 5 mg/kg subcutaneously for 5 days. Body weight dynamics was registered, total protein and albumin level, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, gamma-glutamyl transferase activity was determined in blood plasma, uric acid and urea content – in blood plasma and urine. Several favourable effects of the combination of goutweed tincture and metformin were seen, namely, the reduction in plasma ALT activity and increase in urea clearance as well as normalization of ALP activity. In contrast to metformin, goutweed tincture limited the dexamethasone-induced increase in plasma albumin concentration and decreased De Ritis ratio. Dexamethasone tended to increase renal uric acid excretion, metformin led to the further increment, in the groups receiving goutweed tincture this value was unchanged, but, proceeding from the normal values of uricemia, extrarenal mechanisms of the influence on purine metabolism were possible in these animals. All of the investigated preparations did not influence on plasma AST activity and caused further decrease in body weight that was reduced under the influence of dexamethasone. In-depth research of the interaction of goutweed tincture with metformin is expedient.
Abstract: The interest in the preclinical studies of the efficacy and safety of herbal drugs combinations with antidiabetic medicines increases. Aegopodium podagraria L. (goutweed) is a plant widely used in traditional medicine. The tincture obtained from its aerial part is characterized by antihyperglycemic, lipid-lowering, hepatoprotective and nephroprotec...
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