The increasing reliance on plant-based healthcare products, including herbal medicines and dietary supplements, emphasizes the global significance of traditional remedies. Aloe Vera with 400 reported species, has been widely used herbal remedy for health practices worldwide. Despite its extensive historical use and therapeutic reputation, recent studies have raised concerns about potential adverse effects, challenging the notion of Aloe Vera as a universally safe functional food material. This study aimed to analyze the phytochemical composition of whole leaf extracts at various maturation stages of Aloe Vera and assess their antibacterial effect against Staphylococcus aureus. The qualitative phytochemical analysis revealed a concentration gradient, with older leaves exhibiting higher concentrations compared to medium and young leaves, suggesting a dynamic maturation-related variation. The antibacterial assay demonstrated age-dependent inhibitory activities, with older leaves displaying the highest, medium leaves following, and young leaves exhibiting the least inhibition. A consistent minimum inhibitory concentration of 12.5 mg/ml was observed across all leaf ages. These findings stress the need for cautious Aloe Vera consumption, especially in rural communities where whole-leaf extraction is prevalent, as recent studies have reported adverse effects and potential health risks associated with certain compounds. Safer alternatives, and regulating consumption practices are recommended, emphasizing sustainable practices to maximize plant benefits and minimize waste.
| Published in | Journal of Diseases and Medicinal Plants (Volume 10, Issue 4) |
| DOI | 10.11648/j.jdmp.20241004.11 |
| Page(s) | 60-68 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Aloe Vera, Medicinal Plants, Staphylococcus aureus, MBC, MIC
| [1] | A. Robert, I. Isaac, and W. Joel, “Phytochemistry and Antibacterial Activity of Platycerium coronarium (Tanduk Rasa Fern) Leaf Extracts,” J. Dis. Med. Plants, vol. 9, no. 4, pp. 137–147, Nov. 2023, |
| [2] | World Health Organization (WHO), “WHO Traditional Medicine Strategy 2014-2023,” World Heal. Organ., pp. 1–76, 2013. |
| [3] | G. M. John, D. L. Hershman, L. Falci, Z. Shi, W.-Y. Tsai, and H. Greenlee, “Complementary and alternative medicine use among US cancer survivors,” J. Cancer Surviv., vol. 10, no. 5, pp. 850–864, 2016, |
| [4] | U. Nandal and R. L. Bhardwaj, “Aloe vera: A valuable wonder plant for food, medicine and cosmetic use - a review,” Int. J. Pharm. Sci. Rev. Res., vol. 13, pp. 59–67, Mar. 2012. |
| [5] | M. H. Radha and N. P. Laxmipriya, “Evaluation of biological properties and clinical effectiveness of Aloe vera: A systematic review,” J. Tradit. Complement. Med., vol. 5, no. 1, pp. 21–26, 2015, |
| [6] | S. Ishaque, A. Arshad, M. Haider, and F. Fatima, “Biological and Clinical Sciences Research Journal,” Biol. Clin. Sci. Res. J., pp. 1–9, 2021. |
| [7] | E. V. Christaki and P. C. Florou-Paneri, “Aloe vera: A plant for many uses,” J. Food, Agric. Environ., vol. 8, no. 2, pp. 245–249, 2010. |
| [8] | S. Rahman, P. Carter, and N. Bhattarai, “Aloe Vera for Tissue Engineering Applications,” J. Funct. Biomater., vol. 8, no. 1, p. 6, 2017, |
| [9] | G. Anywar, P. Tugume, and E. K. Kakudidi, “A review of Aloe species used in traditional medicine in East Africa,” South African J. Bot., vol. 147, pp. 1027–1041, 2022, |
| [10] | L. K. Keter and P. C. Mutiso, “Ethnobotanical studies of medicinal plants used by Traditional Health Practitioners in the management of diabetes in Lower Eastern Province, Kenya,” J. Ethnopharmacol., vol. 139, no. 1, pp. 74–80, 2012, |
| [11] | P. O. Otieno, “A review on antiplasmodial potential and quantification of aloin and aloe-emodin in aloe vera.,” 2022, Busitema University. |
| [12] | A. Jangra, G. Sharma, S. Sihag, and V. Chhokar, “The dark side of miracle plant-Aloe vera: a review,” Mol. Biol. Rep., vol. 49, no. 6, pp. 5029–5040, 2022, |
| [13] | D. Hekmatpou, F. Mehrabi, K. Rahzani, and A. Aminiyan, “The effect of aloe vera clinical trials on prevention and healing of skin wound: A systematic review,” Iran. J. Med. Sci., vol. 44, no. 1, pp. 1–9, 2019. |
| [14] | X. Guo and N. Mei, “Aloe vera: A review of toxicity and adverse clinical effects,” J. Environ. Sci. Heal. Part C, vol. 34, no. 2, pp. 77–96, Apr. 2016, |
| [15] | A. Manilal et al., “In vitro antibacterial activity of medicinal plants against biofilm-forming methicillin-resistant Staphylococcus aureus: efficacy of Moringa stenopetala and Rosmarinus officinalis extracts,” Heliyon, vol. 6, no. 1, p. e03303, 2020, |
| [16] |
L. Damian and S. Paţachia, “Method for Testing the Antimicrobial Character of the Materials and Their Fitting To the Scope,” Bull. Transilv. Univ. Braşov, vol. 7, no. 56, pp. 37–44, 2014, [Online]. Available:
http://webbut.unitbv.ro/BU2013/2014/Series_I/BULETIN I/Damian N.pdf |
| [17] | K. S. Banu and L. Cathrine, “General Techniques Involved in Phytochemical Analysis,” Int. J. Adv. Res. Chem. Sci., vol. 2, no. 4, pp. 25–32, 2015, [Online]. Available: |
| [18] |
M. A. Wani, S. P. Ceo, U. P. Livestock, O. Prakash, and S. Prasad, “Qualitative phytochemical analysis of various parts of bamboo (Bambusa balcooa) for possible therapeutic usages in bovine reproductive disorders,” ~ 217 ~ J. Pharmacogn. Phytochem., vol. 8, no. 1, pp. 217–221, 2019, [Online]. Available:
https://www.phytojournal.com/archives/2019/vol8issue1/PartD/7-6-459-332.pdf |
| [19] | M. Balouiri, M. Sadiki, and S. K. Ibnsouda, “Methods for in vitro evaluating antimicrobial activity: A review,” J. Pharm. Anal., vol. 6, no. 2, pp. 71–79, 2016, |
| [20] | M. Benkova, O. Soukup, and J. Marek, “Antimicrobial susceptibility testing: currently used methods and devices and the near future in clinical practice,” J. Appl. Microbiol., vol. 129, no. 4, pp. 806–822, Oct. 2020, |
| [21] | G. C. Omojate, F. O. Enwa, A. O. Jewo, and C. O. Eze, “Mechanisms of Antimicrobial Actions of Phytochemicals against Enteric Pathogens – A Review,” J. Pharm. Chem. Biol. Sci., vol. 2, no. 2, pp. 77–85, 2014. |
| [22] | V. Brezáni and K. Šmejkal, “Secondary metabolites isolated from the genus Eucalyptus,” Chemistry, vol. 1, 2006. |
| [23] | K. Sebei, F. Sakouhi, W. Herchi, M. L. Khouja, and S. Boukhchina, “Chemical composition and antibacterial activities of seven Eucalyptus species essential oils leaves,” Biol. Res., vol. 48, pp. 1–5, 2015, |
| [24] | R. HO and O. PE, “Antimicrobial Potency of Methanolic Leaf Extracts from Selected Medicinal Plants against Staphylococcus aureus,” J. Med. Microbiol. Diagnosis, vol. 5, no. 1, pp. 1–4, 2016, |
| [25] | V. Ikpe, C. S. Eze, P. Mbaoji, and P. E. Joshua, “Phytochemical analysis and antifungi activity of aloe vera leaves,” Bio-Research, vol. 15, no. 1, p. 974, 2019, |
| [26] | K. Thu, Y. Y. Mon, T. A. Khaing, and O. M. Tun, “Study on Phytochemical Properties, Antibacterial Activity and Cytotoxicity of Aloe vera L.,” Int. J. Biotechnol. Bioeng., vol. 7, no. 5, pp. 285–289, 2013. |
| [27] | H. Omer, M. Abakar, S. E. A. Bakhiet, and R. S. M. Abadi, “Antimicrobial activity and minimum inhibitory concentration of Aloe vera sap and leaves using different extracts,” J. Pharmacogn. Phytochem., vol. 6, no. 3, pp. 297–302, 2017. |
| [28] | P. Danish, Q. Ali, M. Hafeez, and A. Malik, “Antifungal and Antibacterial Activity of Aloe Vera Plant Extract,” Biol. Clin. Sci. Res. J., vol. 2020, no. 1, pp. 1–8, 2020, |
| [29] | G. G. Yebpella, M. M. Adeyemi Hassan, C. Hammuel, A. M. Magomya, A. S. Agbaji, and E. M. Okonkwo, “Phtyochemical screening and comparative study of antimicrobial activity of Aloe vera various extracts,” African J. Microbiol. Res., vol. 5, no. 10, pp. 1182–1187, 2011, |
APA Style
Robert, A., Isaac, I. (2024). Phytochemical Analysis and Antibacterial Activity of Aloe Vera Leaf Extracts Across Different Leaf Ages. Journal of Diseases and Medicinal Plants, 10(4), 60-68. https://doi.org/10.11648/j.jdmp.20241004.11
ACS Style
Robert, A.; Isaac, I. Phytochemical Analysis and Antibacterial Activity of Aloe Vera Leaf Extracts Across Different Leaf Ages. J. Dis. Med. Plants 2024, 10(4), 60-68. doi: 10.11648/j.jdmp.20241004.11
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Download@article{10.11648/j.jdmp.20241004.11,
author = {Alule Robert and Isabirye Isaac},
title = {Phytochemical Analysis and Antibacterial Activity of Aloe Vera Leaf Extracts Across Different Leaf Ages
},
journal = {Journal of Diseases and Medicinal Plants},
volume = {10},
number = {4},
pages = {60-68},
doi = {10.11648/j.jdmp.20241004.11},
url = {https://doi.org/10.11648/j.jdmp.20241004.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20241004.11},
abstract = {The increasing reliance on plant-based healthcare products, including herbal medicines and dietary supplements, emphasizes the global significance of traditional remedies. Aloe Vera with 400 reported species, has been widely used herbal remedy for health practices worldwide. Despite its extensive historical use and therapeutic reputation, recent studies have raised concerns about potential adverse effects, challenging the notion of Aloe Vera as a universally safe functional food material. This study aimed to analyze the phytochemical composition of whole leaf extracts at various maturation stages of Aloe Vera and assess their antibacterial effect against Staphylococcus aureus. The qualitative phytochemical analysis revealed a concentration gradient, with older leaves exhibiting higher concentrations compared to medium and young leaves, suggesting a dynamic maturation-related variation. The antibacterial assay demonstrated age-dependent inhibitory activities, with older leaves displaying the highest, medium leaves following, and young leaves exhibiting the least inhibition. A consistent minimum inhibitory concentration of 12.5 mg/ml was observed across all leaf ages. These findings stress the need for cautious Aloe Vera consumption, especially in rural communities where whole-leaf extraction is prevalent, as recent studies have reported adverse effects and potential health risks associated with certain compounds. Safer alternatives, and regulating consumption practices are recommended, emphasizing sustainable practices to maximize plant benefits and minimize waste.
},
year = {2024}
}
TY - JOUR T1 - Phytochemical Analysis and Antibacterial Activity of Aloe Vera Leaf Extracts Across Different Leaf Ages AU - Alule Robert AU - Isabirye Isaac Y1 - 2024/12/23 PY - 2024 N1 - https://doi.org/10.11648/j.jdmp.20241004.11 DO - 10.11648/j.jdmp.20241004.11 T2 - Journal of Diseases and Medicinal Plants JF - Journal of Diseases and Medicinal Plants JO - Journal of Diseases and Medicinal Plants SP - 60 EP - 68 PB - Science Publishing Group SN - 2469-8210 UR - https://doi.org/10.11648/j.jdmp.20241004.11 AB - The increasing reliance on plant-based healthcare products, including herbal medicines and dietary supplements, emphasizes the global significance of traditional remedies. Aloe Vera with 400 reported species, has been widely used herbal remedy for health practices worldwide. Despite its extensive historical use and therapeutic reputation, recent studies have raised concerns about potential adverse effects, challenging the notion of Aloe Vera as a universally safe functional food material. This study aimed to analyze the phytochemical composition of whole leaf extracts at various maturation stages of Aloe Vera and assess their antibacterial effect against Staphylococcus aureus. The qualitative phytochemical analysis revealed a concentration gradient, with older leaves exhibiting higher concentrations compared to medium and young leaves, suggesting a dynamic maturation-related variation. The antibacterial assay demonstrated age-dependent inhibitory activities, with older leaves displaying the highest, medium leaves following, and young leaves exhibiting the least inhibition. A consistent minimum inhibitory concentration of 12.5 mg/ml was observed across all leaf ages. These findings stress the need for cautious Aloe Vera consumption, especially in rural communities where whole-leaf extraction is prevalent, as recent studies have reported adverse effects and potential health risks associated with certain compounds. Safer alternatives, and regulating consumption practices are recommended, emphasizing sustainable practices to maximize plant benefits and minimize waste. VL - 10 IS - 4 ER -
Department of Biological Sciences, Faculty of Science, Kyambogo University, Kyambogo, Uganda
Department of Biological Sciences, Faculty of Science, Kyambogo University, Kyambogo, Uganda
